Chunlai CHEN Ph.D.
Associate Professor
1999-2003 Peking University B.Sc.
2003-2008 Peking University Ph.D.
2008-2015 University of Pennsylvania Postdoc
2015-2018 School of Life Sciences Tsinghua University Assistant Professor
2018-now School of Life Sciences Tsinghua University Associate Professor
Research Area:
1) Develop cutting-edge single molecule fluorescence methods.
2) Dynamics and molecular mechanisms of target searching of proteins on DNA.
3) Molecular mechanisms of CRISPR proteins in target searching and cleavage.
Selected publications
1. Jingfei Xu(#), Lingyun Zhao(#), Sijia Peng(#), Huiying Chu(#), Rui Liang, Meng Tian, Philip P. Connell, Guohui Li (*), Chunlai Chen (*) and Hong-Wei Wang (*), (2021) Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination, Nucleic Acids Research, 10.1093/nar/gkab1141
2. Xiaolin Niu(#), Ruirui Sun(#), Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2021) Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Nat Commun, 10.1038/s41467-021-26616-x
3. Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu (*) and Chunlai Chen (*), (2021) Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chem Sci, 12, 12776-12784
4. Ming-Jie Han(#), Qing-tao He(#), Mengyi Yang(#), Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kong-kai Zhu, Changxiu Qu, Fan Yang, Cheng Hu, Xuzhen Guo, Dawei Zhang, Chunlai Chen (*), Jin-peng Sun (*) and Jiangyun Wang (*), (2021) Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chem Sci, 12, 9114-9123
5. Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*) and Chunlai Chen (*), (2020) Phase separation at the nanoscale quantified by dcFCCS, PNAS, 117(44):27124-27131
6. Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*) and Chunlai Chen (*), (2020) Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS, 117(36):21889-21895
7. Man Pan (#), Qingyun Zheng (#), Shan Ding (#), Lujia Zhang (#), Qian Qu, Tian Wang, Danning Hong, Yunjing Ren, Lujun Liang, Chunlai Chen (*), Ziqing Mei (*), and Lei Liu (*), (2019) Chemical Protein Synthesis-Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angew. Chem. Int. Ed, 58(9):2627-2631
8. Bo Wu, Haibo Zhang, Ruirui Sun, Sijia Peng, Barry S Cooperman, Yale E Goldman (*), and Chunlai Chen (*), (2018) Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots, Nucleic Acids Research, 46(18): 9736-9748
9. Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382
10. Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2018) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, 9(10): 890-895
11. Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885
Contact information
email :chunlai@tsinghua.edu.cn
