Jijie Chai Ph.D.
Professor
B.S. Chemical Engineering, 1983-1987, Dalian Institute of Light Industry, China
M.S, Applied Chemistry, Research Institute of Petroleum Processing,
Ph.D. Medicinal Analysis, 1994-1997, Peking Union Medical College, China
2009.03-present Tsinghua University, School of Life Sceinces
2004-2009.03 National Institute of Biological Sciences, Beijing, China
1999-2004 postdoctoral fellow, Department of Molecular Biology, Princeton University, USA
1997-1999 postdoctoral fellow, Institute of Biophysics, Chinese Academy of Sciences
Research interest
The main interest of the laboratory is focused on structural and functional studies of biologically and medically important macromolecules, mainly through protein crystallography, in couple with biochemical, biological and other approaches, to gain insights into structure-function relationship of these macromolecules. Our research program embraces, but is not limited to, collaborations with the groups within National Institute of Biological Sciences, where several joint projects have been initiated. Specifically, one line of the research will be focused on the elucidation the molecular mechanisms underling transcription regulation by covalent histone modifications, especially histone methylation. Another project we are currently interested in is about pathogen-host interaction. The long-term goal of our lab is to investigate the structure-function relationship of protein involved in these two fields.
Selected publications
1. S. Huang, A. Jia, W. Song, G. Hessler, Y. Meng, Y. Sun, L. Xu, H. Laessle, J. Jirschitzka, S. Ma, Y. Xiao, D. Yu, J. Hou, R. Liu, H. Sun, X. Liu, Z. Han, J. Chang, J. Parker and J. Chai Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity. Science. 7 July 2022
2. A. Jia, S. Huang, W. Song, Wang J, Y. Meng, Y. Sun, L. Xu, H. Laessle, J. Jirschitzka, J. Hou, T. Zhang, W. Yu, G. Hessler, E. Li, S. Ma, D Yu, J. Gebauer, U. Baumann, X. Liu, Z. Han, J. Chang, J. Parker and J. Chai. TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity. Science, 7 July 2022
3. D. Yu, W. Song, EYJ. Tan, L. Liu, Y. Cao, J. Jirschitzka, E. Li, E. Logemann, C. Xu, S. Huang, A. Jia, X. Chang, Z. Han, B. Wu, P. Schulze-Lefert, J. Chai. TIR domains of plant immune receptors are 2',3'-cAMP/cGMP synthetases mediating cell death. Cell. 2022 May 13: S0092-8674(22)00530-X.
4. M. Huang, X. Zhang, G. A. Toh, Q. Gong, J. Wang, Z. Han, B. Wu, F. Zhong*, J. Chai*, Structural and biochemical mechanisms of NLRP1 inhibition by DPP9. Nature 592, 773-777 (2021).
5. G. Bi, M. Su, N. Li, Y. Liang, S. Dang, J. Xu, M. Hu, J. Wang, M. Zou, Y. Deng, Q. Li, S. Huang, J. Li, J. Chai*, K. He*, Y. H. Chen*, J. M. Zhou*, The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling. Cell, (2021).
6. S. Ma, D. Lapin, L. Liu, Y. Sun, W. Song, X. Zhang, E. Logemann, D. Yu, J. Wang, J. Jirschitzka, Z. Han, P. Schulze-Lefert*, J. E. Parker*, J. Chai*, Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. Science 370, (2020).
7. Y. Xiao, M. Stegmann, Z. Han, T. A. DeFalco, K. Parys, L. Xu, Y. Belkhadir, C. Zipfel*, J. Chai*, Mechanisms of RALF peptide perception by a heterotypic receptor complex. Nature 572, 270-274 (2019)..
8. J. Wang, J. Wang, M. Hu, S. Wu, J. Qi, G. Wang, Z. Han, Y. Qi, N. Gao, H. W. Wang*, J. M. Zhou*, J. Chai*, Ligand-triggered allosteric ADP release primes a plant NLR complex. Science 364, (2019).
9. J. Wang, M. Hu, J. Wang, J. Qi, Z. Han, G. Wang, Y. Qi, H. W. Wang*, J. M. Zhou*, J. Chai*, Reconstitution and structure of a plant NLR resistosome conferring immunity. Science 364, (2019).
10. J. Wang, H. Li, Z. Han, H. Zhang, T. Wang, G. Lin, J. Chang, W. Yang*, J. Chai*, Allosteric receptor activation by the plant peptide hormone phytosulfokine. Nature 525, 265-268 (2015).
11. Z. Hu, Q. Zhou, C. Zhang, S. Fan, W. Cheng, Y. Zhao, F. Shao, H. W. Wang, S. F. Sui*, J. Chai*, Structural and biochemical basis for induced self-propagation of NLRC4. Science 350, 399-404 (2015).
12. Y. Sun, L. Li, A. P. Macho, Z. Han*, Z. Hu, C. Zipfel, J. M. Zhou*, J. Chai*, Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science 342, 624-628 (2013).
13. Z. Hu, C. Yan, P. Liu, Z. Huang, R. Ma, C. Zhang, R. Wang, Y. Zhang, F. Martinon, D. Miao, H. Deng, J. Wang, J. Chang, J. Chai*, Crystal structure of NLRC4 reveals its autoinhibition mechanism. Science 341, 172-175 (2013).
14. T. Liu, Z. Liu, C. Song, Y. Hu, Z. Han, J. She, F. Fan, J. Wang, C. Jin, J. Chang, J. M. Zhou*, J. Chai*, Chitin-induced dimerization activates a plant immune receptor. Science 336, 1160-1164 (2012).
15. J. She, Z. Han, T. W. Kim, J. Wang, W. Cheng, J. Chang, S. Shi, J. Wang, M. Yang, Z. Y. Wang, J. Chai*, Structural insight into brassinosteroid perception by BRI1. Nature 474, 472-476 (2011).
Contact information
E-mail：chaijj@tsinghua.edu.cn
