Jun-Jie (Gogo) Liu Ph.D.
Assistant Professor
2007-2011, B.S., School of Life Sciences, Sichuan University
2011-2016, Ph.D., Peking University-Tsinghua University-NIBS Joint Ph.D. Program
2016-2018, Postdoctoral Fellow, MBIB Division, Lawrence Berkeley National Laboratory
2018-2020, Postdoctoral Fellow, Department of Molecular and Cell biology, UC Berkeley
2018-2020, Life Science Research Fund Fellow Sponsored by Pfizer
2020,10- Tenure-Track Assistant Professor, School of Life Sciences, Tsinghua University
● Research Interest：
The Liu lab uses multidisciplinary approaches to study the following topics:
（1） Design and development of new gene-editing tools
（2） Mechanism study about RNA-involved nuclease machinery
（3） Functional and structural study of human disease related LncRNA and the development of new therapy method.
● Selected Publications:
1. Tsuchida CA, Zhang SY, Doost MS, Zhao YQ, Wang J, O’Brien E, Fang H, Cheng-Ping Li, Li DY, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JJG*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Molecular Cell (2022).
2.Yang MY, Sun RR, Deng PJ, Yang YZ, Wang WJ, Liu JJG*, Chen CL*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem. Sci (2021). (Co-corresponding author)
3. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA. Target preference of Type III A CRISPR-Cas complexes at the transcription bubble. Nature Communications,10(1), 3001(2019). (Co-first author)
4. Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knot GJ, Harrington L, Al-Shayeb B, Wanger A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 566,218-223 (2019).
5. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Denomy JB, Doudna JA. Temperature-responsive competitive inhibition of CRISPR-Cas9. Molecular Cell, 1016-016(2018). (Co-first author)
6. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA. Structures of the CRISPR genome integration complex. Science, 357, 1113-1118 (2017). (Co-first author)
7. Shing J#, Jiang F#, Liu JJ#, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA. Disabling Cas9 by an anti-CRISPR DNA mimic. Science Advances, 3(7): e1701620(2017). (Co-first author)
8. Ma M#, Liu JJ#, Li Y, Huang Y, Ta N, Chen Y, Fu H, Ye MD, Ding Y, Huang W, Wang J, Dong MQ, Yu L, Wang HW. Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and-C2. Cell Research, 27,989-1001(2017). (Co-first author)
9. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Dong MQ, Huang N & Wang HW, CryoEM structure of yeast cytoplasmic exosome complex, Cell Research, 26, 822-837(2016).
10. Liu JJ, Bratkowski MA, Liu XQ, Niu CY, Ke AL & Wang HW, Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM, Nature Structural & Molecular Biology, 21, 95–102 (2014).
● Contact info：
E-mail：junjiegogoliu@tsinghua.edu.cn
Website：http://gogolab.life.tsinghua.edu.cn
Tel：+86-010-62770270 (lab & office)
Address：A401, Bio-Medical Building, School of Life Sciences, Tsinghua University, Beijing, 100084, China
